Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 724-2

724-2

ANTIBACTERIAL ACTIVITY of Amburana cearensis RESIN EXTRACT ON Staphylococcus spp. CAPRINE MASTITIS ISOLATES

Autores:

Priscila Izidro de Figueirêdo (UFRPE - UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO, RECIFE – PE ) ; Nayara Andreo (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE) ; Gabriel Fernando Costa de Souza (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE) ; Betilde de Matos Silva (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE) ; Ana Beatriz Carvalho Ferreira (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE) ; Danillo Sales Rosa (UFRPE - UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO, RECIFE – PE ) ; Tania Maria Sarmento da Silva (UFRPE - UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO, RECIFE – PE ) ; Mateus Matiuzzi da Costa (UNIVASF - UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO, PETROLINA, PE)

Resumo:

Caprine mastitis consequences include damage to milk quality and production, economic losses, health of herds, in addition to public health risks. Inappropriate use of antimicrobials contributes to bacterial resistance of agents involved in the disease etiology, such as Staphylococcus spp. Plant extracts have been considered a potential alternative herbal medicine. In this sense, Amburana cearensis (Allemão) A.C. Smith, a Caatinga biome woody species, has been the target of studies about its chemical characterization and pharmacological properties, however, it is incipient the investigations about the resin potential. Thus, the aim was to evaluate the antibacterial activity of A. cearensis resin extract against different species of Staphylococcus isolated from caprine mastitis. The study was carried out at the Laboratory of Animal Microbiology and Immunology at Federal University of Vale do São Francisco, Petrolina - PE. Resin extract was obtained by mortar maceration, solubilized with MeOH (methanol) in an ultrasound bath until complete exhaustion. An aqueous extract solution at 25,000 µg/mL was prepared with 5 % of cremophor and 5 % of dimethylsulfoxide. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) are determined by broth microdilution method using Mueller-Hinton medium, making up concentrations from 12,500 to 97.61 µg/mL. For the assay, one S. aureus, one S. chromogenes and one S. epidermidis isolates were used and also the American Type Culture Collection standard strains: S. aureus ATCC 25923, methicillin sensitive and S. aureus ATCC 33591 methicillin-resistant. The assay was carried out in technical and biological triplicate and the results were expressed as arithmetic mean. A. cearensis resin extract showed antibacterial activity with MIC and MBC equal to 781.25 µg/mL for all evaluated species. This activity may be related to the phenolic compounds present in the A. cearensis resin extract, pointing a potential bactericidal effect against isolates which cause mastitis in goats. Thus, information about resin antibacterial activity, in addition to adding another biological value for A. cearensis, also demonstrates that the extract may have promising pharmacological application, as an herbal medicine, contributing to antimicrobials resistance mitigation.

Palavras-chave:

Antimicrobial, Bacteria, Cumaru, Phytotherapeutic

Agência de fomento:

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE).